Composite

Part:BBa_K4286203:Design

Designed by: Bingru Feng   Group: iGEM22_SZU-China   (2022-10-09)


PCaMV-prb1-6xHis-TNOS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1946
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1046
    Illegal NgoMIV site found at 1091
    Illegal NgoMIV site found at 1385
    Illegal NgoMIV site found at 1499
    Illegal AgeI site found at 2015
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 66
    Illegal BsaI site found at 1232
    Illegal BsaI.rc site found at 1109


Design Notes

The protease PRB1 has been proven to be an effective biocontrol agent against a variety of plant fungal pathogens. In our design, PRB1 will be overexpressed in Trichoderma atroviride to enhance its inhibitory effect on Rhizoctonia solani.


Source

PRB1 comes from Trichoderma atroviride IMI 206040.

References